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Proteins in the human intraocular lens (IOL) capsule may cause opacification of the posterior capsule after cataract surgery. In this study, the effect of (NH(4))(2)SO(4) and (NH(4))(2)PO(4) on the proteins in the IOL capsule was investigated. A clear floating zone of cells was obtained in a mixed culture of IOL cells and the rat iris pigment epithelium (IPE) cells for 30 days with (NH(4))(2)SO(4) and (NH(4))(2)PO(4). An IOL capsule was obtained from a patient with a senile cataract. The proteins in the capsule were extracted with different salts. The protein concentration in the salt solution was adjusted to 5% of that in the original mixed culture of the IOL cells and IPE cells, and the pellets obtained were resuspended in 0.5 M Tris-HCl, pH 7.8. The effect of the various salts on the proteins in the IOL capsule was studied in relation to the amount of (NH(4))(2)SO(4) or (NH(4))(2)PO(4) used. An electroelution technique was used to separate proteins. The proteins obtained from the IOL capsule were subjected to SDS-polyacrylamide gel electrophoresis. The gel was stained with Coomassie blue and the protein bands were quantified by scanning densitometry. The proteins in the IOL capsule were soluble in 0.5 M Tris-HCl, pH 7.8. The amount of the proteins increased as the concentration of salts was increased. When 0.8 M (NH(4))(2)SO(4) was added, the fraction of alpha-crystallin in the total protein increased. With (NH(4))(2)SO(4) added at 0.8 M, the concentration of the proteins in the IOL capsule increased as much as approximately 30% compared with that in the capsule without (NH(4))(2)SO(4), but the amount of beta-crystallin decreased. 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