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Another study indicated that a combination of bortezomib and capecitabine led to significantly longer survival than bortezomib monotherapy in an orthotopic mouse model of pancreatic cancer and syngeneic mouse breast cancer \[[@B80]\]. Furthermore, an autophagy inhibitor, 3-methyladenine, has been found to sensitize non-small-cell lung cancer cells to docetaxel \[[@B81]\]. Surprisingly, the autophagy inhibitor 3-methyladenine has been reported to increase drug-induced cell death in colorectal cancer cells \[[@B82]\]. In summary, the complexity of the process makes it difficult to precisely determine the contribution of autophagy to the chemotherapy response. Therefore, if the role of autophagy during chemotherapy is not sufficiently clear, it could be valuable to test whether a combination of autophagy inhibitors and chemotherapy would enhance the treatment response. Targeting autophagy for cancer therapy ====================================== As presented in this review, accumulating evidence suggests that autophagy plays a protective role in cancer. Thus, induction of autophagy could be a potential strategy for cancer therapy. Although the specific mechanism is unclear, inhibition of autophagy could enhance the cytotoxic effect of anticancer therapies. Unfortunately, the first clinical trials for targeting autophagy showed disappointing results. Autophagy inhibitors, 3-methyladenine and hydroxychloroquine, reduced the accumulation of proteins in melanoma cell lines and patient samples. However, these inhibitors failed to cause any significant tumor growth reduction in mice \[[@B83],[@B84]\]. Another study with 3-methyladenine demonstrated similar results. 3-Methyladenine was found to induce autophagy, but it did not affect the growth of tumor xenografts \[[@B85]\]. Thus, inhibiting autophagy is not a good strategy for cancer treatment. 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